For the altarpiece of the Church of the Most Holy Annunciation, Leonardo chose the Madonna with the baby, Saint Anne and the baby John.
The shapes and poses of the figures depicted in the figure can be compared to ancient sculptures. Presumably Leonardo visited Rome in 1501, and there he could witness the beginning of excavations in Tivoli at the villa of Emperor Hadrian, built around 118 AD. e., and see the statues of the muses found in antique gardens.
This is written by Marani in his book, published in 2003. During the excavations in Rome, the ancient sculptures of Apollo Belvedere and the Roman marble copy of the statue of David, made from the ancient Greek bronze original, were discovered. It was found around 1499-1500. The figures depicted by Leonardo look like antique statues.